Biotransformation Studies of Agricultural Nitrogen Pollutants in Keduang Watershed

Pujiastuti Peni, Narimo Narimo, Roesleini J Putri

Abstract


The present study seeks to examine nitrogen biotransformation of agricultural wastewater carried out by nitrosomonas and nitrobacter into Ammonia (N-NH3), Nitrite (N-NO2), and Nitrate (N-NO3) in Keduang watershed. Natural capability of the bacteria is necessary to find out to monitor assimilative capacity of the waterbody towards pollutants. Grab sampling technique was applied in agricultural land and Keduang watershed in reference to Indonesian National Standard (SNI) 6989.59:2008. Meanwhile, analysis of N-NO2 was based on Indonesian National Standard (SNI) 06-6989.09-2004, N-NO3 on SNI 6989.79-2011, and N-NH3 on SNI 06-6989.30-2005. The nitrosomonas and nitrobacter were isolated and identified on NA medium considering methods of Capuccino and Sherman (2005). Afterwards, characterization of colony morphology variants was determined, and both gram stain and biochemical test were conducted. A number of 48.8 nitrosomonas colonies/100 mL were identified in samples of agricultural wastewater, which enable to transform Ammonia (N-NH3) of 0.1390 mg/L into Nitrite (N-NO2) of 0.0632 mg/L. Meanwhile, a number of 330 nitrobacter colonies/ 100 mL are capable of transforming Nitrite (N-NO2) into Nitrate (N-NO3) of 0.2168 mg/L. In conclusion, there is a positive relationship between nitrosomonas in transforming Ammonia into Nitrite and nitrobacter in converting Nitrite into Nitrate. Nitrogen pollutants of the agricultural wastewater in Keduang watershed are able to be reduced by both nitrosomonas and nitrobacter.

Keywords


Agricultural wastewater; Biotransformation; Nitrogen; Nitrobacter; Nitrosomonas

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References


Agustiningsih, D., Sasongko, S.B.& Sudarso. 2012.Analisis Kualitas Air dan Beban Pencemaran Berdasarkan Penggunaan Lahan di Sungai Blukar Kabupaten Kendal.Proceeding in The National Seminar of “Pengelolaan SDA & Lingkungan”, Semarang, Indonesia. 11 September2012.

Anshori, Imam, 2008, Kajian Sistemik Untuk Pemandu Arah Kebijakan Mengurangi Sedimentasi Waduk Wonogiri, Thesis Program Magister Studi Pembangunan Sekolah Arsitektur, Perencanaan dan Pengembangan Kebijakan, ITB.

Aswadi Muhammad, 2006. Pemodelan Fluktuasi Nitrogen (Nitrit) pada Aliran Sungai Palu. Jurnal SMARTek, 4 (2), 112-125.

Cappucino, James G, Natalie Sherman, 2014, Microbiology: a laboratory manual/James G. Cappucino, Natalie Sherman, 10th ed. Pearson, www.pearsonhighered.com

Casali, J. R. Gimenez, J. Diez, J. Álvarez-Mozos, J. D.V. de Lersundi, M. Goni, M.A. Campo, Y. Chahor, R. Gastesi, J. Lopez, 2010. Sediment production and water quality of watersheds with contrasting land use in Navarre (Spain). Agricultural Water Management 97 pp. 1683–1694

Durn, Z.V.H., Francia, M.J.R., Gracia, T.I., Rodriques, ap.c.r.,Martinez, R.A. & Cuadros, T.S. 2012. Runoff & Sediment Yield from a Small Watershed in Southeastern Spain (Lanjar n): implication for water quality. Hidrological Sciences Journal, 57 (8), 1610-1625.

EPA-US, 2009, 2009 Hazardous Waste Report, Instructions and Form, United State Environmental Protection Agency, EPA Form 8700-13 A/B, November 2009.

Kementrian Lingkungan Hidup, 2010, Status Lingkungan Hidup Indonesia 2010, ISBN 978-602-8358: 39-2

Kiding Agustina, Khotimah Siti, Linda Riza,.2015, Karakterisasi dan Kepadatan Bakteri Nitrifikasi pada Tingkat Kematangan Tanah Gambut yang Berbeda Di Kawasan Hutan Lindung Gunung Ambawang Kabupaten Kubu Raya, Jurnal Protobiont, Vol. 4 (1), 2015: 17-21

Manuba, I.B. Putra, 2009, Cemaran Pestisida Karbamat Dalam Air Danau Buyan Buleleng Bali, Jurnal Kimia 3(1), ISSN 1907-9850, Januari 2009:47-54

Masniawati, A., As’adi, A. 2017. Isolasi Bakteri Nitrifikasi pada Rhizosfer Tanaman Padi Aromatik Lokal (Oryza sativa L.) di Kabupaten Toraja Sulawesi Selatan. http:// scholar google.co.id.

Marganof, 2007. Model Pengendalian Pencemaran Perairan di Danau Maninjau Sumatra Barat, Laporan Hasil Penelitian Sekolah Pascasarjana IPB Bogor.http://www.damandiri.or.id/ile/marganoipb

Maridi, Sutarno, Shalihuddin Djalal Tandjung, Ari Handono Ramelan. 2012. Fighting Through Community Participation Based on Vegetative Conservation Approach of Wonogiri Reservoir Sedimentation Sub - Watershed of Keduang. Surakarta : Universitas Sebelas Maret. Journal of Environment and Earth Science. ISSN 2224-3216 (Paper) ISSN 2225-0948 (Online). Vol 2, No.7, 2012 0948 (Online)

Mayo W. Aloyce, Hanal E Emmanual, 2014, Dynamics of Nitrogen Transformation and Removal in a Pilot High Rate Pond. Journal of Water Resource and Protetection, 2014, 6, 433-445.

Presiden Republik Indonesia. 2001. “Peraturan Pemerintah Republik Indonesia Nomor 82 Tahun 2001 Tentang Pengelolaan Kualitas Air dan Pengendalian Pencemaran Air”. Jakarta

Pujiastuti, P., Sumardiyono, Mutiah, Syahriyati. 2015. Pola Distribusi Pencemar Nitrogen dan Fosfor Non Point Source Sub Das Wuryantoro ke Waduk Gajah Mungkur Wonogiri.Paper presented in SNaTKII II (National Seminar on Chemical, Industrial and Information Technologies), Surakarta, Indonesia.10 October2015.

Ramadhani, Rezky. 2015. “Distribusi Bakteri Nitrifikasi (Nitrosomonas dan Nitrobacter) di Muara Sungai Tallo Kota Makasar”. Skripsi. Makasar : Universitas Hasanudin

Saha Mousumi, Sarkar Agnisvar and Bandhophadhyay. 2013. Developmant of Molucular Identification of Nitrifing Bacteria in Water Bodies of East Kolkata Wetland, West Bengal, Journal Bioremidiation & Biodegradation, 2013, 5;1, http://dx.doi.org/ 10.4172/2155-6199.1000211. ISSN: 2155-6199 JBRBD, an open access journal.

Shen, Q.R., Ran, W., Cao, H. 2003.Mechanisms of Nitrite Accumulation Occurring in Soil Niytrification.Chemosphere Journal, 50(6), 747-753.

Standar Nasional Indonesia 6989.79:2011 – Bagian 79: Cara Uji Kadar Nitrat (NO3-N) dengan Alat Spektrofotometer UV – Visible Secara Reduksi Kadmium.2011: BSN.

Standar Nasional Indonesia 06-6989.59:2008 tentang Air dan Air Limbah – Bagian 59: Metode pengambilan contoh air limbah. 2008: BSN.

Standar Nasional Indonesia 06-6989.30-2005 tentang Air dan Air Limbah – Bagian 30 : Cara Uji Kadar Amonia dengan Spektrofotometri secara Fenat. 2005 : BSN

Standar Nasional Indonesia 6989.09-2004 tentang Air dan Air Limbah – Bagian 9: Cara Uji Nitrit (NO2-N) secara Spectrofotometri. 2004: BSN.

Sudarno. 2012. Perkembangan Biofilm Nitrifikasi di Fixed Bed Reactor pada Salinitas Tinggi.Jurnal Presipitasi, 9 (1).

Suriadarma Adi, 2011, Dampak Beberapa Parameter Faktor Fisika Kimia Terhadap Kualiitas Lingkungan Perairan Wilayah Pesisir Karawang-Jawa Barat. Jurnal Riset, Geologi dan Pertambangan, Vol/ 21, No. 2, Juni 2011. Puslit Geoteknologi. LIPI.

Titiresmi &Nida, S.2006.Teknologi Biofilter untuk Pengolahan Limbah Ammonia.Jurnal Teknik Lingkungan PTL-BPPT, 7(2), 173-179.

Xia Yu, H. Lingguang, Xu Ligang. 2011.Characteristics of Diffuse Source N Pollution in Lean River Catchment.Procedia Environmental Sciences, 10, 2437 – 2443




DOI: http://dx.doi.org/10.21771/jrtppi.2018.v9.no2.p21-29

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