Pengaruh Konsentrasi Substrat terhadap Kadar Gliserol dan Konstanta Michaelis (Km) pada Hidrolisa CPO secara Enzimatis

Ageng Priatni

Abstract


The aim of this research is to know the effect of substrate concentration on enzymatic hydrolysis reaction of CPO. CPO was hydrolized by water and catalized by lipase enzymes. Amount of water was varied of 50 grams; 100 grams;  150 grams  and  200  grams.  The result  showed  that  substrate concentration had effect on glycerol content and Michaelis Constant (Km).13,28 % glycerol content and   2,04x 10-2M (gmo/lml) Michaelis Constancy (Km) were produced at 625,86 mg/ml substrate concentration

Keywords


substrate, hydrolyze, glycerol, Michaelis Constant(Km).

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DOI: http://dx.doi.org/10.26578/jrti.v3i6.1417

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Akreditasi S2 Vol.10 No.1 th 2016 s/d Vol.14 No.2 th 2020. p-ISSN : 1978-6891, e-ISSN : 2541-5905. 

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