Characterization of Ethanol Extract from Katumpangan Air Herbs (Peperomia pellucida L. Kunth

Marissa Angelina, Puteri Amelia, Muchammad Irsyad, Lia Meilawati, Muhammad Hanafi


Peperomia pellucida L. Kunth known as “Katumpangan Air” is a potential medicinal plants used traditionally for uric acid, rheumatic, headache, stomachache, antimicrobial. This study aims to establish  the non-specific  and specific parameters of ethanol extract from Katumpangan air (EKA). EKA was collected from three different growth places: South Tangerang (6.833%), Bogor (7.8%) and Yogyakarta (13.125%). The results show that organoleptic extract characterization from three places were same: thick, blackish green brown, bitter and have a characteristic odor. The average content of water-soluble compounds was within range 7.39-13.29%, ethanol-soluble 15.33-16.68%, flavonoid total 3.807-4.244%. The range of moisture content was 12.25-16.34%, ash total 1.21-2.78%, acid insoluble ash 0.19-1.62%, drying shrinkage 21.62-24.98%, specific gravity 1.0010-1.0034 g/mL for the non-specific parameter. Microbial contamination testing results 0.61x103-1.13x103 coloni/g, the contaminant testing mold/yeast 0.1x102-1.7x102 coloni/g, while lead metal 0.15-0.18 mg/kg, cadmium 0-0.11 mg/kg and arsenic <0.005µg/kg.


non-specific parameters; Peperomia pellucida L. Kunth; specific parameters

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Atmoko, Tri & Ma’ruf, Amir. (2009). Uji Toksisitas dan Skrining Fitokimia Ekstrak Tumbuhan Sumber Pakan Orangutan Terhadap Larva Artemia salina L Jurnal Penelitian Hutan dan Konservasi Alam. 6(1), 37-45.

Abere, T. A., Agoreyo, F. O., & Eze, G. I. (2013). Phytochemical, Antimicrobial and Toxlicological evaluation of the leaves of Peperomia Pellucida (L.) HBK (Piperaceae). Journal of Pharmaceutical and Allied Sciences. 9(3), 1637-1652.

Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M. H., ElSohly, M. A., & Khan, I. A. (2014). Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: A comparative study. Evidence-Based Complementary and Alternative Medicine, 2014.

Ciulei, I. (1982). Methodology for analysis of vegetable drugs. Practical manual on the industrial utilisation of medicinal and aromatic plants. Romania: Center Building.

Depkes Republik Indonesia. (2006). Monografi Ekstrak Tumbuhan Obat Indonesia. volume 2. Jakarta: BPOM Republik Indonesia.

Depkes Republik Indonesia. (2007). Kebijakan Obat Tradisional Nasional. Jakarta: Departemen Kesehatan Republik Indonesia.

Depkes Republik Indonesia. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan Republik Indonesia.

Hans-Jorg Bart & Stephan Pilz. (2011). Industrial Scale Natural Products Extraction. First Edition Germany: Wiley-VCH Verlag GmbH & Co. KgaA.

Khan, A., Rahman, M., & Islam, M. S. (2010). Isolation and Bioactivity of a Xanthone Glycoside from Peperomia pellucida. Life Sci and Med Res, 2010, 1-10.

Majumder, Pulak., Abraham, Priya & Satya, V. (2011). Ethno-medicinal, Phytochemical and Pharmacological review of an amazing medicinal herb Peperomia pellucida (L.) HBK. Research Journal of Pharmaceutical, Biological and Chemical. 2(4), 358-364.

Majumder, Pulak. (2012). Evaluation of Taxochemical Standardization and Quality Control Parameter of Peperomia pellucid: A multi valuable medicinal herb. Journal of Pharmaceutical and Scientific Innovation.

Okoh, O. O., Sadimenko, A. A., & Afolayan, A. J. (2007). The effects of age on the yield and composition of the essential oils of Calendula officinalis. J Appl Sci. 7(23), 3806-3810.

Ooi, D. J., Iqbal, S., & Ismail, M. (2012). Proximate composition, nutritional attributes and mineral composition of Peperomia pellucida L.(Ketumpangan Air) grown in Malaysia. Molecules. 17(9), 11139-11145.

Purba, Ritson & Nugroho D. S. (2007). Analisis Fitokimia dan Uji Bioaktivitas Daun kaca (Peperomia pellucida (L.) Kunth). Jurnal Kimia Mulawarman. 5(1), 5-8.

Saifudin, Aziz., Rahayu, Viesa.,Teruna & Hilwan Yuda. (2011). Standardisasi Bahan Obat Alam. Edisi Pertama. Yogyakarta: Graha Ilmu.

Soetarno, S., & I.S. Soediro. (1997). Standardisasi Mutu Simplisia dan Ekstrak Bahan Obat Tradisional. Bandung: Presidium Temu Ilmiah Nasional Bidang Farmasi.

Wei, L. S., Wee, W., Siong, J. Y. F., & Syamsumir, D. F. (2011). Characterization of anticancer, antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract. Acta Medica Iranica. 49(10), 670-674.



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