Search Region of Origin Honey Bee A. mellifera in Indonesia Region Using Mitochondrial DNA intergenic cox1/cox2

Mohamad Rusdi Hidayat


Apis mellifera is a favourite honey bee for the beekeepers throughout many countries. This species comprise of 24 subspecies. Based on phylogeography and morphometric evidences, these subspecies have been grouped into four lineage; namely the African (A), Western and Northern Europe (M), Southeastern Europe (C), and Near Eastern (O). Apis mellifera have been imported to Indonesia since 1972, and mostly from Australia. However, until recently there are no data about the A. mellifera subspecies and the origin. Therefore the objective of this research is to determine the lineage of A. mellifera in Indonesia based on mtDNA intergenic region between cox1/cox2 genes. In this region there are two DNA fragments, P and Q fragnant, that can be used to determine the A. mellifera lineage. The methodology used consist of samples collection, DNA isolation, DNA amplification, DNA restriction using DraI enzyme, DNA sequencing, and DNA alignment using Clustal X and MEGA spftwares. DNA fragment amplified by using E2 and H1 primer revealed a 863 bp. Digestion of the region with the DraI restriction enzyme revealed one haplotype, which consist of five DNA fragments. Based on DNA sequences and DNA alignment, A. mellifera in Indonesia was homologue with the C lineage. Its subspecies is A. m. ligustica that lived natively in Italy, they were imported to Indonesia from Australia


A. mellifera; intergenic region cox1/cox2; mtDNA

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Arias MC, Sheppard C.A., 1996, Molecular phylogenetics of honeybee subspecies (Apis mellifera L.) inferred from mitochondrial DNA sequence. Mol. Phylogenet and Evol. 5:557-566.

Borror DJ, Triplehorn CA, Johnson N.F., 1982, An Inroduction to the Study of Insects. Ohio: Saunders College Publ.

Clarke KE, Oldroyd BP, Javier J, Quezada- Euan G, Rinderer TE., 2001, Origin of honeybees (Apis mellifera L.) from the Yucatan peninsula inferred from mitochondrial DNA analysis. Mol. Ecol. 10:1347-1355.

Crozier RH & Crozier YC., 1993, The mitochondrial genome of the honeybee Apis mellifera: complete sequence and genome organization. Genetics 133: 97-117.

Cornuet JM, Garnery L, Solignac M., 1991, Putative origin and function of the intergenic region between cox1 and cox2 of Apis mellifera L. mitochondrial DNA. Genetics 1128: 393-403.

Engelsdorp VD., 2009, Colony Collapse Disorder: A Descriptive Study. PLoS ONE Vol. 4, 8.

Franck P, Garnery L, Solignac M, Cornuet JM., 2000a, Molecular confimation of a fourth lineage in honeybees in the Near East. Apidologie 31(2):167-180.

Frank P et al., 2000b, Hybrid origin of honeybee from Italy (Apis mellifera ligustica) and Sicily (A. m. sicula). Mol. Ecol. 9: 907-921.

Garnery L, Mosshine EH, Oldroyd BP, Conuet JM., 1995, Mitochondrial DNA variation in Marrocan and Spannish honeybee population. Mol. Ecol. 4: 465-471.

Gojmerac WL., 1983, Bees, Beekeeping, Honey and Pollination. Connecticut: AVI Publishing.

Kumar S, Tamura K and Nei M., 2004, MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. 5: 150-163.

Koulianos S & Crozier RH., 1997, Mitochondrial sequence of Australian commercial and feral honeybee strains, A. mellifera L. (Hymenoptera: Apidae), in the context of species worldwide. Aus. J. Entomol, 36: 359-364.

Marlina I., 2004, Karakterisasi intron 2 gen inositol 1, 4, 5-triphosphate receptor (itpr) lebah Apis mellifera [skripsi]. Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.

Mattila HR et al., 2007, Genetic Diversity in Honey Bee Colonies Enhances Productivity and Fitness. Science 317, 362

Moritz RFA., 1983, Inbreeding Effects in Flight Muscle Mitochondria of Apis mellifera L. Brazil. J. Genet. VI, 1, 59-70.

Özdil F, Yildiz MA, dan Hall HG., 2009, Molecular characterization of Turkish honey bee populations (Apis mellifera) inferred from mitochondrial DNA RFLP and sequence results. Apidologie 40: 570–576.

Ruttner F., 1988, Biogeography and Taxonomy of Honeybeeys. Berlin: Springer.

Sambrooks J, Fritsch EF, Maniatis T., 1989, Molecular Cloning a Laboratory Manual. Ed ke-2. New York: Cold Spring Harbor Pr.

Sukartiko B., 1986, Evaluasi budidaya lebah madu di Indonesia. Prosiding Lokakarya Pembudidayaan Lebah Madu untuk Peningkatan Kesejahteraan Masyarakat; Sukabumi, 20-22 Mei 1986, Jakarta, hlm 97-111.



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