ISOLASI DAN IDENTIFIKASI KAPANG ENDOFIT DARI TANAMAN KUNYIT (Curcuma longa L.) SEBAGAI PENGHASIL ANTIOKSIDAN

Tiwit Widowati, Bustanussalam Bustanussalam, Harmastini Sukiman, Partomuan Simanjuntak

Abstract


Endophyte fungi are microbe that living inside the plant tissue without harming the host plant. Endophyte fungi can produce secondary metabolite which can be used as antioxidant, anticancer and antimicobes compound. Endophyte fungi can be found in many plants especially herbs such as turmeric (Curcuma longa L). The aims of this study are to isolate and identify endophyte fungi from stem of C. longa L. which is potential as an antioxidant producer. The endophyte fungi isolated from turmeric stem were 12 isolates. Antioxidant activity was assayed using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) showed that isolate K.Cl.Sb.B1 produced the highest inhibition value (78,81%). Based on molecular identification, the isolate K.Cl.Sb.B1 was Colletotrichum sp.

Keywords: Curcuma longa L., endophyte fungi, identification antioxidant

 

ABSTRAK

Kapang endofit merupakan mikroba yang terdapat di dalam jaringan tanaman tanpa membahayakan tanaman inang. Kapang endofit mampu menghasilkan metabolit sekunder yang dapat dimanfaatkan sebagai senyawa antioksidan, antikanker dan antimikroba. Kapang endofit dapat ditemukan pada berbagai jenis tanaman terutama tanaman obat seperti kunyit (Curcuma longa L). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kapang endofit dari batang tanaman kunyit yang berpotensi sebagai penghasil antioksidan. Kapang endofit yang diisolasi dari batang tanaman kunyit diperoleh 12 isolat. Uji antioksidan menggunakan 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) menunjukkan bahwa isolat K.Cl.Sb.B1 menghasilkan nilai inhibisi tertinggi (78,81%). Berdasarkan identifikasi molekuler, isolat K.Cl.Sb.B1 merupakan Colletotrichum sp.

Kata kunci: Curcuma longa L., identifikasi antioksidan, kapang endofit


Keywords


Curcuma longa L.; identifikasi antioksidan; kapang endofit

Full Text:

PDF

References


Arau´jo, W. L., Marcon, J., Maccheroni Jr, W., van Elsas, J. D. & Azevedo, J. L. (2002). Diversity of Endophytic Bacterial Populations and Their Interaction with Xylella fastidiosa in Citrus Plants. Applied and Environmental Microbiology. 68(10), 4906-4914. DOI: 10.1128/AEM.68.10.4906-4914.2002.

Bougatef, A., Muhammed, H., Rafik, B., Imen, L., Yosra, T. E. & Moncef, N. (2009). Antioxidant and free radical-scavenging activities of smooth hound (Mustelus) muscle protein hydrolysates by gastrointestinal proteases. Food Chemical. 114, 1198-1205.

Chapla, V. M., Zeraik, M. L., Leptokarydis, I. H., Silva, G. H., Bolzani, V. S., Young, M. C. M., Pfenning, L. H. & Araujo, A. R. (2014). Antifungal Compounds Produced by Colletotrichum gloeosporioides, an Endophytic Fungus from Michelia champaca. Molecules. 19, 19243-19252. doi:10.3390/molecules191119243.

DDBJ. (2015). Blast. DNA Data Bank of Japan (DDBJ). http://blast.ddbj.nig.ac.jp/.

Ezra, D., Hess, W. M. & Strobel G. A. (2004). New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus. Microbiology. 150, 4023-4031. doi: 10.1099/mic0.27334-0.

Gangadevi, V. & Muthumary, J. (2008). Isolation of Colletotrichum gloeosporioides, a novel endophytic taxol-producing fungus from the leaves of a medicinal plant, Justicia gendarussa. Mycologia Balcanica. 5, 1-4.

Hall, Tom. (2013). BioEdit. Ibis Biosciences Carlsbad, CA 92008. http://www.mbio.ncsu.edu/BioEdit/ bioedit.html.

Harper, J. K., Arif, A. M. & Ford E. J. (2003). Pestacin: a 1,3-dihydro isobenzofuran from Pestalotiopsis microspora possessing antioxidant and antimycotic activities. Tetrahedron. 59(14), 2471-2476.

Hemtasin, C., Kanokmedhakul, S., Kanokmedhakul, K., Hahnvajanawong, C., Soytong, K., Prabpai, S. & Kongsaeree, P. 2011. Cytotoxic Pentacyclic and tetracyclic aromatic sesquiterpenes from Phomopsis archeri. J. Nat. Prod. 74(4), 609-613.

Hiraishi, A., Kamagata, Y. & Nakamura, N. (1995). Polymerase chain reaction amplification and restriction fragment length polymorphism analysis of 16S rRNA genes from methanogens. Journal of Fermentation Bioengineering. 79, 523-529.

Hung, P. Q. & Annapurna, K. (2004). Isolation and characterization of endophytic bacteria in soybean (Glycine sp.). Omonrice. 12, 92-101.

I-Nan Chen, I. C., Chang. C. C., Ng C. C., Wang, C. Y., Shyu, Y. T. & Chang, T. L. (2008). Antioxidant and Antimicrobial Activity of Zingiberaceae Plants in Taiwan. Plant. Foods Hum. Nutr. 63, 15–20.

Isaka, M., Chinthanom, P., Boonruangprapa, T., Rungjindamai, N. & Pinruan, U. (2010). Eremophilanetype sesquiterpenes from the fungus Xylaria sp. BCC 21097. J. Nat. Prod. 73, 683–687.

Maehara, S., Ikeda, M., Haraguchi, H., Kitamura, C., Nagoe, T., Ohashi, K. & Shibuya, H. (2011). Microbial conversion of curcumin into colorless hydroderivatives by the endophytic fungus Diaporthe sp. associated with Curcuma longa. Chem Pharm Bull. 59(8), 1042-1044.

Molyneux, P. (2004). The use of the stable free radical diphenylpycryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanarin Journal of Science Technology. 26(2), 211-219.

Nath, A., Pathak, J. & Joshi, S. R. (2014). Bioactivity assessment of endophytic fungi associated with Centella asiatica and Murraya koengii. Journal of Applied Biology & Biotechnology. 2(05), 006-011. DOI: 10.7324/ JABB.2014.2502.

NCBI. (2016). Blast. National Center for Biotechnology Information. U.S. National Library of Medicine. http://blast.ncbi.nlm.nih.gov/Blast.cgi.

O`Donnell, K. (1993). Fusarium and its near relatives. In: Reynolds, D.R. & Taylor, J.W. (eds), The fungal holomorph: Mitotic, meiotic and pleomorphic specification in fungal systematic (pp. 225-233). CAB International, Wallingford.

Pawle, G. & Singh, S. K. (2014). Antioxidant Potential of Endophytic Fungus Colletotrichum spesies isolated from Polygala elongata. International Journal of Pharma and Bio Sciences. 5(3), 313-319.

Rodriguez, R. & Redman, R. (2008). More than 400 million years of evolution and some plant still can’t make it on their own: plant stress tolerance via fungal symbiosis. Journal of Experiment Botany. 59(5), 1109-1114. DOI:10.1093/jxb/erm342.

Saowaluck, B. & Yingyong, P. (2009). Essential Oil and Antioxidant Activity of Cassumunar Ginger (Zingiberaceae: Zingiber montanum (Koenig) Link ex Dietr.) Collected from Various Parts of Thailand. Kasetsart Journal Natural Science. 43, 467-475.

Strobel, G.A. & Daisy, B. (2003). Bioprocessing for microbial endophytes and their natural products. Microbiology and Molecular Biology Revievs. 67(4), 491-502. DOI: 10.1128/MMBR.67.4.491-502.2003.

Taechowisan, T., Lu, C., Shen, Y. & Lumyong, S. (2005). Secondary metabolites from endophytic Streptomyces aureofaciens CMUAc130 and their antifungal activity. Microbiology. 151, 1691-1695. DOI:10.1099/mic0.27758-0.

Tianpanich, K., Prachya, S., Wiyakrutta, S., Mahidol, C., Ruchirawat, S. & Kittakoop, P. (2011). Radical Scavenging and Antioxidant Activities of Isocoumarins and a Phthalide from the Endophytic Fungus Colletotrichum sp. Journal of Natural Product. 74, 79-81.

Tomita, F. (2003). Endophytes in Southeast Asia and Japan: their taxonomic diversity and potential applications. Fungal Diversity. 14, 187-204.

White, T. J., Bruns, T. D., Lee, S. B. & Taylor, J. W. (1990). Amplification and direct sequencing of fungal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J. J. & White, T. J. (eds). PCR protocols (pp. 315-322) Academic, San Diego.


Refbacks

  • There are currently no refbacks.


BIOPROPAL Industri

Published by :

Institute for Industrial Research and Standardization (Baristand Industri) in Pontianak

Agency for Industrial Research and Development, Ministry of Industry 

Jl. Budi Utomo No. 41 Pontianak, West Kalimantan, Indonesia

Tel / Fax : +62 561 881393, 881533

email      : biopropal.industri@gmail.com

 

BIOPROPAL Industri indexed in: 

Hasil gambar untuk gambar doajHasil gambar untuk gambar google scholar