Tiwit Widowati, Bustanussalam Bustanussalam, Harmastini Sukiman, Partomuan Simanjuntak


Endophyte fungi are microbe that living inside the plant tissue without harming the host plant. Endophyte fungi can produce secondary metabolite which can be used as antioxidant, anticancer and antimicobes compound. Endophyte fungi can be found in many plants especially herbs such as turmeric (Curcuma longa L). The aims of this study are to isolate and identify endophyte fungi from stem of C. longa L. which is potential as an antioxidant producer. The endophyte fungi isolated from turmeric stem were 12 isolates. Antioxidant activity was assayed using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) showed that isolate K.Cl.Sb.B1 produced the highest inhibition value (78,81%). Based on molecular identification, the isolate K.Cl.Sb.B1 was Colletotrichum sp.

Keywords: Curcuma longa L., endophyte fungi, identification antioxidant



Kapang endofit merupakan mikroba yang terdapat di dalam jaringan tanaman tanpa membahayakan tanaman inang. Kapang endofit mampu menghasilkan metabolit sekunder yang dapat dimanfaatkan sebagai senyawa antioksidan, antikanker dan antimikroba. Kapang endofit dapat ditemukan pada berbagai jenis tanaman terutama tanaman obat seperti kunyit (Curcuma longa L). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kapang endofit dari batang tanaman kunyit yang berpotensi sebagai penghasil antioksidan. Kapang endofit yang diisolasi dari batang tanaman kunyit diperoleh 12 isolat. Uji antioksidan menggunakan 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) menunjukkan bahwa isolat K.Cl.Sb.B1 menghasilkan nilai inhibisi tertinggi (78,81%). Berdasarkan identifikasi molekuler, isolat K.Cl.Sb.B1 merupakan Colletotrichum sp.

Kata kunci: Curcuma longa L., identifikasi antioksidan, kapang endofit


Curcuma longa L.; identifikasi antioksidan; kapang endofit

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