Aspiyanto Aspiyanto, Agustine Susilowati, Yati Maryati, Hakiki Melanie


The condition of Stirred Filtration Cell (SFC) was used as reference to a large-scale process conditions (modules) on the separation offermented inulin fibers by Lactobacillus acidophillus. Inulin hydrolyzate as biomass was produced  from inulinase hydrolysis stage by inulinase enzyme from Aspergillus clavatus-CBS5. Separation of inulinfiber aims to obtain inulin fiber through a microfiltration (MF) membrane 0.45 μm at room temperature, 400 rpm stirrer cycle and 40 psia for 0, 30, 60, 90 and 120 min. The results showed that best separation time was 120 minutes based on optimal CBC (cholesterol binding capacity) which fermented inulin fiber concentrate was produced with a total sugar concentration of 105.21 mg/mL, total solids 2.11%, total fiber 23.36%, total acid 6.66% (dry weight), 4.05 mg of dissolved protein/mL and CBC 13.781 mg/g. MF membrane increased the CBC by 23.4% compared to no separation process.

Keywords: cholesterol binding capacity, inulin fiber, microfiltration membrane, permeate, retentate


Kondisi Sel Filtrasi Berpengaduk (SFB) digunakan sebagai acuan menuju kondisi proses skala besar (modul) terhadap pemisahan serat inulin terfermentasi oleh Lactobacillus acidophillus. Hidrolisat inulin yang digunakan sebagai biomassa dihasilkan dari tahapan hidrolisa inulin oleh enzim inulinase dari kapang Aspergillus clavatus-CBS5. Pemisahan serat inulin bertujuan untuk mendapatkan serat inulin melalui membran mikrofiltrasi (MF) 0,45 µm pada temperatur ruang, kecepatan putaran pengaduk 400 rpm dan tekanan 40 psia selama 0, 30, 60, 90 dan 120 menit. Hasil penelitian menunjukkan bahwa waktu pemisahan yang lama dapat menahan dan meningkatkan padatan total, serat total, asam total, protein terlarut dan kemampuan pengikat kolesterol (KPK) tetapi menurunkan gula total dalam retentat. Membran mikrofiltrasi melewatkan dan menurunkan gula total, protein terlarut dan KPK tetapi meningkatkan padatan total, serat total, asam total dalam permeat. Berdasarkan KPK optimal, waktu pemisahan terbaik dicapai setelah 120 menit. Pada kondisi ini dihasilkan konsentrat serat inulin terfermentasi dengan konsentrasi gula total 105,21 mg/mL, padatan total 2,11%, serat total 23,36%, asam total 6,66% (berat kering), protein terlarut 4,05 mg/mL dan KPK 13,781 mg/g. Membran MF mampu meningkatkan KPK 23,4% dibandingkan tanpa  menggunakan proses pemisahan.

Kata kunci:          kemampuan pengikat kolesterol, membran mikrofiltrasi, permeat, retentat, serat inulin


cholesterol binding capacity; inulin fiber; microfiltration membrane; permeate; retentate

Full Text:



Akin, O., Temelli, F. & Sefa, K. (2012). Membrane applications in functional foods and nutraceuticals. Crit. Rev. Food Sci. Nutr., 52, 347-349. doi: 10.1080/10408398.2010 .500240.

Alles, M.J.L., Tessaro, I.C. & Norena, C.P.Z. (2015). Concentration and Purification of Yacon (Smallanthus sonchifolius) Root Fructooligosaccharide Using Membrane Technology. June, 53(2), 190-200. doi: 10.17113/ftb.53.02.15. 3766.

Amicon Bioseparation. (2008). Stirred Ultrafiltration Cell. Laboratory Catalogue of Amicon Bioseparation. Millipore Corporation, Bedford, U.S.A.

Baker, R. (2012). Microfiltration, in Membrane Technology and Applications, 3rd edn., John Wiley & Sons Ltd., California, U.S.A., 303.

Cassano, A., Conidi, C., Figueroa, R.R. & Muńoz, R.C. (2015). A Two-Step Nanofiltration Process for the Production of Phenolic-Rich Fractions from Artichoke Aqueous Extracts. International Journal of Molecular Sciences. April, 16(4), 8968. doi: 10.3390/ijms16048968.

Dominguez, A.L., Rodrigues, L.R., Lima, N.M. & Teixeira, J.A. (2013). An Overview of the Recent Developments on Fructooligosaccharide Production and Applications, Food Bioprocess Technol., Springer Science+Business Media, New York. doi: 10.1007/s11947-013-1221-6.

Drioli, E. & Romano, M. (2001). Progress and new perspectives on integrated membrane operations for sustainable industrial growth. Ind. Eng. Chem. Res. 40, 1277-1279. doi: 10.1021/ie000 6209.

Jegatheesan, V., Shu, L., Keir, G. & Phong, D.D. (2012). Evaluating membrane technology for clarification of sugarcane juice. Reviews in Environmental Science and Biotechnology. Print ISSN 1569-1705, Online ISSN 1572-9826, Springer Science+Business Media B.V., Netherlands. June, 11(2), 111. doi:10.1007/s11157-012-9271-1.

Kosasih, W., Pudjiraharti, S., Ratnaningrum, D. & Priatni, S. (2015). Preparation of Inulin from Dahlia Tubers, International Symposium on Applied Chemistry (ISAC 2015). Procedia Chemistry, 16(2015), 190-194. ScienceDirect.

Kumar, P., Sharma, N., Ranjan, R., Kumar, S., Bhat, Z.F., & Jeong, D.K. (2013). Perspective of Membrane Technology in Dairy Industry: A Review, Asian-Australasian Journal of Animal Sciences (AJAS). 26(9), 1350. doi:

Lee, J.Y., Tan, W.S., An, J., Chua, C.K., Tang, C.Y., Fane, A.G. & Chong, T.H. (2016). The potential to enhance membrane module design with 3D printing technology. Journal of Membrane Science. 499, 483. 1016/j.memsci.2015.11.008.

Marchetti, P., Solomon, M.F.J., Szekely, G. & Livingston, A.G. (2014). Molecular Separation with Organic Solvent Nanofiltration: A Critical Review, Chemical Reviews, America Chemical Society (ACS) Publications, October 21, 10738.

Mensink, M.A., Frijlink, H.W., Maarschalk, K.V., Wouter, L.J. & Hinrich, S. (2015). Inulin, a Flexible Oligosaccharide II: Review of Its Pharmaceutical Applications, Carbohydrate Polymers. 134, 418-428.

Claudia, M., Francisco, R. & Maria del, C.D. (2012). Membrane Separation Process in Wastewater Treatment of Food Industry. Benyamin Valdez In Food Industrial Processes: Methods and Equipment, February, ISBN 978-953-307-905-9. Published by Intech, 5100-Rijeka, Croatia, 253. Open Access.

Olsen, O.J. (2000). Operating Manual of DSS LabUnit M20. Danish Separation Systems AS, Nakskov, Denmark, January.

O’Sullivan, T.J., Epstein, A.C., Korchin, S.R. & Beaton, N.C. (1984). Applications of Ultrafiltration in Biotechnology. CEP, January.

Raghavan, S., LaMarta, J., Shah, P., Holmes, J. & Chigurupati, S. (2005). Process for producing a low fat, concentrated meat broth from meat by-products. US Patent US 2005/0170060 A1, August 4, 3.

Saxena, A., Tripathi, B.P., Kumar, M. & Shahi, V.K. (2009). Membrane-based techniques for the separation and purification of proteins: An overview. Adv. Colloid Interface Sci. 145, 5.

Schneeman, B.O. (1986). Dietary Fiber: Physical and Chemical Properties, Methods of Analysis, and Physiological Effects. Food Technol., 40, 104-110.

Susilowati, A. (2013). Karakteristik Enzim Inulinase dari Kapang Endofit Hasil Isolasi Umbi Dahlia Merah (Dahlia sp.) Lokal dan Aplikasinya dalam Perolehan Serat Inulin sebagai Anti Kolesterol. Prosiding Seminar Nasional Sains dan Teknologi ke-4. Fakultas Teknik Universitas Wahid Hasyim Semarang. Semarang 19 Juni 2013, ISBN 978-602-99334-2-0.

Susilowati, A. (2014). Aplikasi Serat Inulin Terfermentasi sebagai Anti Kolesterol. DIPA Tematik Jasa Iptek Tahun Anggaran 2014. Pusat Penelitian Kimia-LIPI, PUSPIPTEK, Serpong.

Tsurumaki, M., Kotake, M., Iwasaki, M., Saito, M., Tanaka, K., Aw, W., Fukuda, S. & Tomita, M. (2015). The application of omics technologies in the functional evaluation of inulin and inulin-containing prebiotics dietary supplementation. Journal of Nutrition & Diabetes. 5(11), 185. doi: 10.1038/ nutd.2015.35.

Younis, K., Ahmad, S. & Jahan, K. (2015). Health Benefits and Application of Prebiotics in Foods, Journal of Food Processing & Technology. ISSN 2157-7110. 6(4), 1. doi: 10.4172/2157-7110. 1000433.



  • There are currently no refbacks.


Published by :

Institute for Industrial Research and Standardization (Baristand Industri) in Pontianak

Agency for Industrial Research and Development, Ministry of Industry 

Jl. Budi Utomo No. 41 Pontianak, West Kalimantan, Indonesia

Tel / Fax : +62 561 881393, 881533

email      :


BIOPROPAL Industri indexed in: 

Hasil gambar untuk gambar doajHasil gambar untuk gambar google scholar